HighPure™ Blood Exosomes Isolation Kit is designed for sEV purification from large-volume samples, such as cell culture medium, urine et.al.
The column IB and IIB are recommended to isolate sEV from no more than 40 ml and 120 ml cell culture medium once and the sample size can easily scale-up dependent on customization.
Relative fast flow velocity is required for the purification procedure, while it can work with our AutoExoPureTM constant flow pump and regular FPLC
The purification by the column is based on a combination of size exclusion and adsorb chromatography and removes more non-EV contamination than the method on the basis of a single separation principle.
The regular maintenance keeps the column staying at good performance.
The purified sEV as the eluent can be concentrated as necessary, e.g. ultrafiltration, absorption centration.
Quick purificationComplete purification of 120ml sample in 8 min
High recovery>90% exosomes derived from the sample are retained.
High purity>99% soluble protein less than 600KD is removed
Instrument CompatibilityCompatible with constant flow pump and FPLC
Flexible specificationCustomization of purification process scale-up
Exosomes Isolation Kit for Cell Culture Medium I 23SBI020102-1
Exosomes Isolation Kit for Cell Culture Medium II 23SBI020102-2
Exosomes Isolation Kit for Cell Culture Medium III 23SBI020102-3
Exosomes Isolation Kit for Blood I 23SBI020202-1
Exosomes Isolation Kit for Blood II 23SBI020202-2
Exosomes Isolation Kit for Blood III 23SBI020202-3
Exosomes Isolation Kit for Urine I 23SBI020301-1
Exosomes Isolation Kit for Urine II 23SBI020301-2
Exosomes Isolation Kit for Urine III 23SBI020301-3
Exosomes Isolation Kit for Urine IV 23SBI020301-4
Exosomes Isolation Kit for Urine V 23SBI020301-5